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・ Restricted-access barrier system
・ Restriction
・ Restriction (Archive album)
・ Restriction (mathematics)
・ Restriction digest
・ Restriction endonuclease BsobI/AvaI
・ Restriction enzyme
・ Restriction fragment
・ Restriction fragment length polymorphism
・ Restriction fragment mass polymorphism
・ Restriction landmark genomic scanning
・ Restriction map
・ Restriction modification system
・ Restriction of Hazardous Substances Directive
・ Restriction point
Restriction site
・ Restriction site associated DNA markers
・ Restriction-modification system
・ Restrictions (album)
・ Restrictions on cell phone use while driving in the United States
・ Restrictions on geographic data in China
・ Restrictions on political parties
・ Restrictions on the import of cryptography
・ Restrictive cardiomyopathy
・ Restrictive dermopathy
・ Restrictive design rules
・ Restrictive flow orifice
・ Restrictive lung disease
・ Restrictive Practices Court
・ Restrictiveness


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Restriction site : ウィキペディア英語版
Restriction site
Restriction sites, or restriction recognition sites, are locations on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes. These are generally palindromic sequences (because restriction enzymes usually bind as homodimers), and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby. For example, the common restriction enzyme EcoRI recognizes the palindromic sequence GAATTC and cuts between the G and the A on both the top and bottom strands, leaving an overhang (an end-portion of a DNA strand with no attached complement) known as a sticky end〔 on each end, of AATT. This overhang can then be used to ligate in (see DNA ligase) a piece of DNA with a complementary overhang (another EcoRI-cut piece, for example). Some restriction enzymes cut DNA at a restriction site in a manner which leaves no overhang, called a blunt end.〔
==References==



抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
ウィキペディアで「Restriction site」の詳細全文を読む



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